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Individual results may vary, as outcomes depend on various factors, including metabolism, and some patients may achieve more or less than the average

After 5 washes, 90-L of TMB substrate solution was added into each well, incubated for 20 min at 37 C

This mixture was then diluted in a 1:1 ratio with a dialysis buffer composed of 20 mM TRIS (pH 7.5), 200 mM NaCl, and 0.5 mM TCEP

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