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The Journal of Physical Chemistry B 119: 3940-3947 (2015) Georgiou-Siafis SK, Tsiftsoglou AS

In physiological validation studies however, the sudden rise of plasma ACTH levels might not only stimulate faster cortisol secretion, but also immediately activate a strong negative feedback loop, possibly resulting in a high but shortened cortisol peak

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The oxygen concentration of Control-A (no antioxidant added) remained stable with only slight, erratic variations that are probably linked to the inherent variability of the analytical procedure