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Fluorescence Microscopy Cells grown on coverslips were fixed with 4% PFA for 30 min at room temperature (RT), washed with PBS, permeabilized with 0.05% saponin, 0.5% glycine in PBS for 5 min and blocked with 2% BSA in PBS (0.05% saponin, 0.5% glycine) for 30 min
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The medication should not be exposed to extreme heat, freezing temperatures, and direct sunlight to maintain its potency
May 20 2020;19(1):48
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