GSH concentration is instead measured by either of two methods: by analysis of GS-NEM conjugates by HPLC in the same sample that is used to measure GSSG or, alternatively, by analysis of GSH by spectrophotometry (GSH recycling method) on one additional sample aliquot that has not been derivatized with NEM
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ROS play a crucial role in the pathogenesis of AD because, by reacting with all macromolecules (lipids, proteins, nucleic acids, and carbohydrates), they promote a chain reaction that leads to damage and cell death
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