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As expected, the fluorescence of the Actinin tension indicator changed from orange to yellow-green in response to blebbistatin (Fig

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Wang Y, Yen FS, Zhu XG, Timson RC, Weber R, Xing C, et al

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Therefore, relatively high laser power is required for confocal imaging, which can consequently cause phototoxicity to live cells, especially with excitation at 405 nm (ref