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The homogenate was centrifuged at 10,000 rpm for 20 min and clear supernatants were collected and stored on ice until the time of the assay
Lipid extracts were dissolved in 100 l infusion mixture consisting of 7.5 mM ammonium acetate dissolved in propanol, chloroform and methanol (4:1:2 (v/v/v))
Avoid heavy drinking to prevent side effects like dizziness and nausea
Pekala J, Patkowska-Sokoa B, Bodkowski R, Jamroz D, Nowakowski P, Lochyski S, Librowski T
Cryo-EM structure of the active, G s -protein complexed, human CGRP receptor
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