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After three washes with PBS, the sections were coverslipped using Vectashield mounting medium with DAPI (Vector, Burlingame, CA)

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The concerns about long-term safety are real and, so far, unresolved

Prepared solutions may be maintained under refrigerated or frozen conditions as required by the research protocol, with aliquoting commonly used to help preserve sample integrity by minimizing repeated freeze-thaw cycles

While supplements and OTC remedies can help significantly, exercise provides a unique mechanical stimulus to the gut that supplements cannot replicate