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Optimization of sample preparation conditions To ensure efficient cell lysis, protein precipitation, and simultaneous stabilization of oxidation-prone thiols at a low pH, three common acidic extraction solutions were evaluated for deproteinization efficiency (Fig

No trials specifically enrolled elderly individuals, those with significant cardiovascular disease, individuals with active cancer, or pregnant/breastfeeding women

The flow rate was 0.3 mL/min, the auto-sampler temperature was maintained at 4 C, and the column compartment was set at 40 C

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Mechanistic research has converged on the VEGF signalling axis