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Then, islets were handpicked and cultured at 37 C and 5% CO 2 in RPMI 1640 media (Thermo Fisher Scientific, #11875093) containing 11 mM glucose and supplemented with 5 g/l BSA fraction V (Roche, #107351080001), 100 units/ml penicillin and 100 g/ml streptomycin (1% P/S) (Thermo Fisher Scientific)

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10.1146/annurev-pathol-030420-105050 24 DrozdN

The publication concluded that Supplementation with a combination of vitamin D and L-cysteine or glutathione precursor, rather than supplementation with vitamin D alone, is beneficial and helps achieve more successful vitamin D supplementation. Another paper by A