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The method involved intracellular oxidation of non-fluorescent dye dihydrorhodamine-(DHR)-123 to the fluorescent rhodamine-123 (R123) caused by free radicals and hydrogen peroxide

PMID: 22817979

Melanotan II produces melanogenesis by activation of the MC1 receptor , whereas its clinically documented sexual effects are thought to be related to its ability to activate the MC4 receptor (though the MC3 is thought to also possibly be involved)