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Khanduri, U., & Sharma, A

Cells were then incubated overnight at 4C with different pairs of the following antibodies: mouse anti H2AX (Millipore cat.# 05-636), rabbit anti ICP8 antibody (kindly provided by prof WT Ruyechan, Univesity of Buffalo, Buffalo, NY, USA), as primary antibodies and Cy2-conjugated donkey anti-rabbit IgG and Alexa Fluor 568 donkey anti-mouse IgG (Molecular Probes, Life Technologies), as secondary antibodies (30 min at room temperature)

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Tissue samples from lean and ob/ob mice were homogenized in TRIzol reagent, followed by RNA extraction using standard protocols

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