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The replacement of the media is essential for removing the CuCl 2 and Cu(OAc) 2 interference before assaying the LDH levels as they are shown to reduce the fluorescence signal read-out within treated wells
Rule of thumb: pick a volume that puts your usual dose somewhere between 520 units on an insulin syringe
This makes supplementation especially important for vegetarians, older adults, and those with digestive disorders or a history of GI surgery
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