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Biopsy processing and organoid culture Fresh biopsies were transferred into 5 mL of 2 mM PBS-EDTA and incubated for 45 min on a rotator in a cold room

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Due to its prolonged half-life, CJC-1295 is believed to induce a prolonged cAMP-mediated activation, which might result in prolonged GH release in some murine models

Notably, older adults with obesity and preserved baseline muscle mass represent a particularly understudied phenotype, and dedicated studies are needed to determine whether this subgroup may differentially benefit from the muscle quality improvements associated with GLP-1 RA therapy

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