Phase 1: Introduction (weeks 1 to 4) Start below your expected long-term concentration
You may be at higher risk if you: Follow a vegan or vegetarian diet Eat very little meat, fish, eggs, or dairy Are over the age of 50 Have digestive conditions affecting absorption Take certain medications, such as metformin or acid-reducing medicines Have had certain stomach or bowel surgery Have pernicious anaemia or absorption problems People in higher-risk groups may benefit from advice, testing, or treatment depending on their symptoms and medical history
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Lenny Macrina: Yeah
Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates