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Incubations with primary antibody (anti-Cystatin C, Sigma, HPA013143, used at 1:100 dilution) were performed at room temperature for 30 min
Guilbaud et al
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These result in newly identified Clic4 as modifier of TGF-beta signalling through its function as stabilizer of p-Smad2 and 3 in the nucleus which is essential for Clic4-mediated growth-arrest and differentiation [37]
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