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doi: 10.1001/jamainternmed.2013.2720 221 KnapenLMvan DalemJKeulemansYCvan ErpNPBazelierMTDe BruinMLet al
When OD600 reached 0.71.2, 1 mM IPTG was added to induce expression followed by overnight incubation at 28 C
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