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20,21 In this approach, androgenetic haploid embryonic stem cells (AG-haESCs) with deletions in the differentially methylated regions of the imprinted loci H19 -DMR and IG -DMR (termed DKO-AG-haESCs) are injected into the oocyte cytoplasm to efficiently produce gene-modified animals, referred to as semi-cloned mice
This is covered in greater detail in our article titled 10 Grams of Citrulline
doi: 10.1016/j.etap.2014.02.013, 178
Across preclinical research, BPC-157 has been reported to have a favorable tolerability profile: a 2021 Frontiers in Pharmacology review noted no reported toxicity in animal studies, with the LD1 (lethal dose for 1% of animals) not reached
(PubMed) Fischer LM, daCosta KA, Kwock L, et al
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