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Although sometimes sterile water can be used multiple times in the 24-hour window, depending on its use

Therefore, in this study, we have used human hepatoma HepG2 cancer cells, a commonly used cell culture model, which has been treated with buthionine sulfoximine (BSO), a GSH depleting agent and N-acetylcysteine (NAC), a precursor of GSH synthesis, to study the implications of altered glutathione metabolism in ASA-induced oxidative stress, redox homeostasis and mitochondrial dysfunctions

In contrast, during chronic or infected wound states, the pH shifts to alkaline levels (pH 79) as a result of microbial activity 53,54

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