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According to the World Health Organization (WHO), more than one billion people worldwide are currently living with obesity, highlighting the scale of the potential treatment population
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ii) Primary culture of satellite cells with 5 ng/ml HGF, which received only the LASSS pre-treatment (at 1:8000, without peroxynitrite treatment), exhibited a cell-proliferation activity (evaluated by mRNA expression levels of MyoD and its counter-regulatory factor myogenin at 48-h and 72-h time-points) comparable to that of the positive control culture treated with 5 ng/ml control HGF without LASSS (just with the solvent
This high correlation coefficient suggests a close agreement and consistency between the modified method and the established assay kit, reinforcing the reliability and validity of the modified GPx-DTNB method
