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Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95

Journal of Gerontology vol

The protective effect of UA against ONOO has been confirmed in the mouse model of MS and spinal cord injury (SCI) [44, 45]

It accomplishes this increase in energy by helping to convert food molecules into energy

doi:10.1007/s10071-014-0794-4