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A 10 l qPCR reaction was amplified in duplicate wells for each sample 4.5 l of cDNA mixture (100 ng cDNA template + rest nuclease-free water) with 5 l iTaq Universal SYBR Green Supermix, and 0.5 l forward and reverse primer mixtures (10 pm)
The research is supported by funding from the National Institute on Aging and the National Institute of Diabetes and Digestive and Kidney Diseases, both part of the National Institutes of Health
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