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Step 5: Label and store Write the following on the vial or on a label you attach to it: the reconstitution date, the concentration (mg/mL), and the discard-by date (typically 28-60 days after reconstitution depending on the medication)

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qPCR was performed with SYBR Green Supermix (TaKaRa) with the protocol as follow: 95 C for 5 min, repeating 40 cycles of 95 C for 5 s and 60 C for 45 s using StepOnePlus real-time PCR system (Applied Biosystem, Foster City, CA, USA)

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