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Following extraction and purification, gas chromatography with flame ionization detection (GC-FID) provides a robust method for this compound quantification through fatty acid methyl ester (FAME) analysis
Nature 550 , 204213 (2017)
In the case of topically applied caffeine, its delivery could be shown after only 5 minutes, but only with open hair follicles
The test does not actually detect the drug itself in most cases
Use the PSPeptides peptide calculator to determine the exact BAC water volume for your desired concentration
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