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The mobile phase comprised of (A) 10 mM ammonium formate in 60% aqueous acetonitrile containing 0.1% formic acid and (B) 10 mM ammonium formate in 9:1 mixture of isopropanol and acetonitrile with a 0.5 mL/min flow rate maintained during a 20 min run

In naturally aged mice and in doxorubicin-treated mice, FOXO4-DRI treatment significantly improved physical fitness, restored fur density, improved renal function, and improved liver function effects attributed to the selective elimination of accumulated senescent cells