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The supernatant was discarded, and isolated cells were separated from debris/myelin using a 70%/37%/30% layered Percoll gradient (Sigma-Aldrich, P1644) with centrifugation at 300 g for 40 min at 18 C without braking

Cell death

Antioxidant and cytoprotective effects of N-acetylcysteine against subchronic oral glyphosate-based herbicide-induced oxidative stress in rats

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