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As de novo synthesis of ceramide is located in the ER, which is absent in erythrocytes, it is thus assumed that erythrocytic ceramide should directly originate from the hydrolysis of sphingomyelin
ER image stack (488 nm channel) first underwent intensity normalization and 3D-gaussian blur (Step 2), followed by thresholded segmentation (Step 3 and 4) via a marker-controlled watershed algorithm (If the ER is tagged with SEC61B, a custom-built filament thresholding algorithm was used in place of marker-controlled watershed algorithm)
Net peptide content confirmed at 27.31mg
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The total number of fat cells in the specific treated pocket is permanently reduced following a successful cycle
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