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The purified peptide 20GIPRG s Nb35 complex at a concentration of 56 mg/mL was mixed with 100 M peptide 20 at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific)

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This is why expiration dates and beyond-use dates matter even when the product looks fine