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Approximately 150 g of digested peptide from each sample was used for purification, which is within the C18 spin columns maximum binding capacity of 100 g (#744101, Harvard Apparatus, Holliston, MA, USA) and then lyophilized before being analyzed on the Orbitrap Fusion Tribrid mass spectrometer coupled with an Ultimate 3000 nano-UPLC system (Thermo Fisher Scientific, Waltham, MA, USA)

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2021;85(6):e413

Additional experimental data point to involvement of MC5R in peripheral signaling, although the precise pathways remain uncertain

In both animal studies and some early human research, activation of this pathway has been associated with reduced food intake and, in some cases, modest weight loss