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The bacterial pellets were resuspended in buffer [50 mM tris (pH 7.5), 500 mM NaCl, 2 mM 2-mercaptoethanol, and 30 mM imidazole]

*Protein targets are based on general guidelines using your weight and activity level

She reported no drop in energy over several months

Decide your activity level Then, to find out your exact ratios based on your daily activity you multiply your BMR by your average activity level based on the following categories: Little or no exercise: 1.2 Light exercise a few times a week: exercise a few times a week: 1.375 Moderate exercise 3-5 times a week: 1.55 Heavy exercise 6-7 times per week: 1.725 So, if the same woman above cycled to work three times a week and attended two yoga classes, she'd be classed as a moderate exercise so would multiply 1348.1 (BMR) x 1.55 (activity level) = 2089.5