Before imaging, the growth medium was removed, then the cells were washed with PBS (2) and imaged in FluoroBrite DMEM
Lancet 2001: 358:8934 13
Menopause, 29 (7), 767-794
Carnitine deficiency impairs a variety of metabolic processes in the liver, including lipid metabolism, gluconeogenesis, ketogenesis, albumin biosynthesis, and ammonia detoxification in the urea cycle [201], and carnitine/acetylcarnitine supplementation can ameliorate a variety of liver diseases [202, 203]
The safest interpretation is that 5-Amino-1MQ is an early-stage research compound with possible metabolic relevance, not a proven replacement for medical treatment, nutrition, exercise, sleep, or provider-guided weight-loss care