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SDSPAGE and western blotting Samples were fractioned by SDSPAGE on 8% gels under reducing conditions, immunoblotted onto nitrocellulose membranes (GE Healthcare) and bands detected by enhanced chemiluminescence (GE Healthcare) onto films developed on a Xograph Compact X5 processor

In elderly patients, due to age-related decline in renal function, the minimum dose of the drug should be used to achieve adequate glycemic control

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