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Moreover, mtDNA release is regarded as a damage-associated molecular pattern in the context of inflammation that can activate TLRs and trigger the production of interferon and cytokines, leading to cell injury (Zhang X

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Embryo and cell immunostaining For section immunostaining, E9.5 embryos were collected, washed with cold PBS, fixed in 4% paraformaldehyde overnight at 4 C, and dehydrated through a graded sucrose series (10%, 20%, and 30%

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Furthermore, IGF-1 promotes the uptake of amino acids, the building blocks of proteins, by muscle cells