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After O/N incubation in either probe, gonads were washed with hybridization buffer three times to remove the excess probe, followed by three washes in PBST

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Check the Certificate of Analysis (COA) for: Identity confirmation (MS data matching expected molecular weight) HPLC purity (greater than 98% is recommended for reliable research work) Fill weight (the actual mass of lyophilized peptide in the vial, which may differ slightly from the nominal amount) Expiration or retest date Using the fill weight from the COA rather than assuming the labeled amount ensures your concentration calculations are accurate

Its shorter half-life creates a more physiological GH release pattern, mimicking the bodys natural pulsatile GH release