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a PDE-5 inhibitor attenuates the hydrolysis of cyclic guanosine monophosphate (cGMP, also called guanosine 3',5'-cyclic monophosphate), thereby increasing the persistence of cGMP, by inhibiting the activity of endogenous type V phosphodiesterase (PDE-5), it being understood that PDE-5 naturally regulates the degradation of cGMP to 5' guanosine monophosphate (5' GMP) in the corpus cavernosum
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The second reaction was initiated through the addition of the remaining 10% (10 l) reaction volume containing GSH and GST and the same plate reader method as used for one-step reactions was applied to follow the reaction progress
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