Samples were centrifuged at 2,500 g for 10 min at 4 C to separate plasma
Several natural compounds have been proposed as interventions for PD including coenzyme Q10, curcumin, resveratrol and omega-3 fatty acids, however these agents have shown limited clinical efficacy, often failing to produce measurable changes in Unified Parkinsons Disease Rating Scale (UPDRS) outcomes or biomarkers of oxidative stress
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Zhao H, Zhang J, Cheng X, Nie X, He B
Subsequently, the cell monolayer was scratched using a 200 L pipette and then washed twice with PBS