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If you experience worsening fatigue, jaundice (yellowing of skin or eyes), abdominal swelling, or confusion, contact your GP urgently, as these may indicate progression of liver disease requiring medical assessment

However, older users often derive greater initial benefit making even partial retention valuable, while younger users might question whether modest improvements justify treatment investment when natural skin quality remains good

Hormonal status: Perimenopausal and postmenopausal women may see a slower response due to estrogen-driven collagen decline

After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min