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Similarly, co-culturing IGF-I / osteoclast precursors, with IGF-I +/+ osteoblast precursors, also produces less (by 50%) multi-nucleated osteoclasts than co-culturing IGF-I +/+ osteoclast precursors with IGF-I +/+ osteoblasts (65), suggesting that the expression of IGF-I in osteoclast precursors is also required to support the full-scale osteoclastogenesis