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The results indicated that TMQ153 exhibited stable binding conformations, with RMSD values showing less fluctuation and remaining consistent throughout the simulation

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Calculate the concentration of the diluted peptide: concentration (mg/mL) = vial capacity (mg) / diluent volume (mL) Use this result to calculate the dosage in mL: dose volume (mL) = target dose (mg) / concentration (mg/mL)

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This can be prevented by derivatizing GSH with the alkylating agent N -ethylmaleimide (NEM) to form GS-NEM before acid deproteination, followed by back-extraction of excess NEM from the deproteinized samples with dichloromethane