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For saturation binding assays, cell lysates (100 L, 25 g of protein for HEK-293 cells and 1020 g of protein for INS-1E cells, quantified by the Bradford Assay) were incubated with the indicated concentrations of [ 3 H]RX821002 (90 min, room temperature)

Thus, astrocytes could contribute to a prion-like spread of misfolded proteins leading to motor neuron loss

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So, if that is the binding buffer that youre using, just add the glutathione to that and it will work great as an elution buffer

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