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The protein sample (250 g protein /30 L) was mixed with 270 L of UA (100 mM TrisHCl (pH 8.5) containing 8 M Urea) in the filter unit, and centrifuged at 14,000 g for 15 min
Optimization of lipophilic metalloporphyrins modifies disease outcomes in a rat model of parkinsonism
2.3.1 Location of Modification The acetylation modification occurs precisely at the thiol group site of the cysteine residue, not at any other position of the molecule
doi: 10.1186/s12906016-09925 197 WangJZhangXLanHWangW
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