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54.6% achieved at least 10% weight loss and 36.0% achieved at least 15%

Thigh injections may produce a slightly lower and later peak compared to abdominal injections

The protein concentration was quantified using a BCA kit, and then SDS-PAGE buffer was added

This simple step prevents most contamination

The gap between theoretical dosing guidelines and practical implementation comes down to three variables most general resources overlook: injection timing relative to endogenous GH pulse windows, reconstitution accuracy affecting per-injection dosing precision, and whether the protocol accounts for receptor desensitisation at higher frequencies