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RINm5F cells were seeded in XF 24-well cell culture microplates (Seahorse Bioscience, Billerica, MA) at 1.0 10 5 cells/well in 100 l complete RPMI-1640 growth medium, and then incubated at 37 C/5% CO 2 for 18-20 h

The article names a personal or family history of medullary thyroid carcinoma, multiple endocrine neoplasia type 2, uncontrolled severe diabetes, severe renal impairment, and concurrent use of other weight-loss medications as contraindications

while in rats it is highly expressed in the C-cells of this organ, there appears to be little detectable expression in non-human primate and human C-cells (85, 88)

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Statistically, these changes were distinct when compared with placebo