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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

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Adding 1 ml to the same vial creates a 5 mg/ml solution

Use a fresh, sterile syringe for each transfer and dispose of needles in a sharps container immediately after use

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