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LCMS/MS analysis was performed using an Advance nanoLC (BrukerMichrom, Auburn, CA, USA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) equipped with a NANOHPLC capillary column C18 (0.075 mm ID 150 mm length, 3 m particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 535% CH 3 CN /0.1% formic acid) at a flow rate of 300 nL/min
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I., & Korkina, L
While the protective effect of folate against NTD is well established, only a few studies have investigated the role of other methyl group donors, including choline and betaine, in the occurrence of NTDs
[PubMed] [Elsevier] Kraus, J
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