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This complexity arises from multifactorial interactions, catalytic degradation kinetics, the hook effect, and tissue-specific E3 ligase expression, that decouple plasma concentration from pharmacological effect

IGF-1 has a longer half-life than growth hormone (about 12-15 hours), so it provides more sustained anabolic signaling even as GH levels fluctuate

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These cells not only retained mitotic capacity, but also maintained a more youthful morphology and gene expression profile, indicating real preservation of cellular youth