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Membranes were blocked and incubated for 1 hour at room temperature with either rabbit monoclonal anti-total Akt (C67E7) (1:1,000, Cell Signaling, #4691), or rabbit monoclonal anti-phospho S473 Akt (D9E) (1:2,000, Cell signaling, #4060)
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Anti-MuRF1 (1:1000, GTX110475) was purchased from GeneTex (Irvine, CA, USA)
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DOI: 10.1056/NEJMoa2032183 VOL
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