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For BrdU staining, the primary BrdU antibody was added together with 0.5 U l 1 DNAse (Sigma, D5025) and 1 mM MgCl 2 (Sigma, M8266) in the blocking solution and incubated for 30 min at room temperature

But it is known that prolonged exposure to oxidative stress can damage cellular components (proteins, lipids and DNA) [13], contribute to cellular aging [14], and play an important role in the pathogenesis of cancer, atherosclerosis, Parkinson, Alzheimers and various chronic diseases such as diabetes mellitus and its complications [15, 16, 17, 18, 19, 20]

The "in chronic kidney disease" language in the D63.1 code title is the technical reason

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