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Similar iterative learning strategies have improved predictive accuracy and design efficiency in synthetic biology and metabolic engineering systems (Carbonell et al., 2019

coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)

Remember that timing is secondary to quality

10.1007/s00709-014-0636-x 5 AnjumN

Its also important that you take a good form of vitamin C