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Verify purity on new lots: For high-stakes studies, request the certificate of analysis for each new lot of BPC-157 or TB-500 before incorporating it into your experimental pipeline

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Cell were washed and placed in normal Ringers buffer (160 mM NaCl, 4.5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES, pH 7.4) or Low Ca 2+ Ringers where CaCl 2 was omitted and 1 mM Ethylenediaminetetraacetic acid (EDTA) added