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Just as BioLock, Biotin Blocking Buffer contains avidin to mask biotin and biotinylated proteins, but the buffer is especially optimized for western blots

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10.1111/dom.15828 44 KatoS.SatoT.FujitaH.KawataniM.YamadaY

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This is due to the presence of hydrogen sulfide gas, derived from proteins containing sulfur